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OTHERPEPTIDE PROFILE

Motilin

Also known as Porcine Motilin, Human Motilin, Gastrointestinal Motilin

Motilin is a 22-amino acid gastrointestinal hormone secreted by Mo/M cells of the upper small intestine during the fasting state. It is the primary regulator of the migrating motor complex (MMC), the coordinated pattern of gastrointestinal contractions that clear the bowel between meals. Motilin is released in approximately 100-minute cycles during fasting and is a key target for prokinetic drug development for gastroparesis and dysmotility.

Last updated April 10, 2026

TL;DR

Quick summary

Motilin is a 22-amino acid GI hormone regulating the migrating motor complex that clears the bowel between meals. Erythromycin acts as a motilin receptor agonist off-label; selective agonists are in gastroparesis trials.

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Overview

Motilin is a 22-amino acid gastrointestinal hormone secreted by Mo/M cells of the upper small intestine during the fasting state. It is the primary regulator of the migrating motor complex (MMC), the coordinated pattern of gastrointestinal contractions that clear the bowel between meals. Motilin is released in approximately 100-minute cycles during fasting and is a key target for prokinetic drug development for gastroparesis and dysmotility.

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Mechanism of action

Motilin binds the motilin receptor (MLNR), a class A GPCR predominantly expressed on smooth muscle cells and interstitial cells of Cajal in the GI tract. Receptor activation via Gq/11 coupling increases intracellular calcium and triggers coordinated smooth muscle contraction, initiating phase III of the migrating motor complex. Erythromycin and motilide antibiotics function as non-peptide motilin receptor agonists and are used off-label as prokinetics. The motilin-ghrelin receptor system shares structural homology, as both peptides derive from related gene families.

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Dosing protocols

PurposeRouteDosageFrequency
GI motility research (animal)intravenous130 pmol/kg/mininfusion per experimental protocol

Dosing information is for educational purposes only. Consult a qualified healthcare professional before using any peptide.

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Research summary

Motilin's role in GI motility has been studied for decades. Clinical interest centers on developing selective motilin receptor agonists for diabetic gastroparesis and chronic intestinal dysmotility. Motilides (erythromycin-derived compounds) show clinical efficacy but are limited by tachyphylaxis. Small-molecule motilin receptor agonists (mitemcinal, camicinal) have advanced to phase II trials for gastroparesis with mixed results. Motilin also coordinates gastric acid secretion and gallbladder emptying during the interdigestive state.[1][2][3][4]

📄This section cites 4 peer-reviewed sources. View all references →
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Evidence grading

Each claimed benefit is graded by the strength of available evidence. Grades reflect study quality, not effect size.

strong
Primary regulator of the migrating motor complexDecades of GI physiology research establish motilin's role in phase III MMC via MLNR on smooth muscle
strong
Erythromycin acts as motilin receptor agonistWell-established non-peptide MLNR agonism used off-label as prokinetic across clinical practice
moderate
Small-molecule agonists showed mixed Phase II gastroparesis resultsMitemcinal and camicinal advanced to Phase II with inconsistent efficacy signals and tachyphylaxis limits
strong
Released in 100-minute fasting cyclesReproducible human plasma kinetics during interdigestive state across multiple physiology studies
preliminary
Coordinates gallbladder emptyingAnimal and limited human data suggest motilin role in interdigestive gallbladder contraction

Strong = multiple RCTs · Moderate = limited trials or observational · Preliminary = animal or in vitro only · Insufficient = anecdotal or no published data

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Side effects

GI cramping (at supraphysiologic doses)
Nausea
Abdominal discomfort

Side effects vary by individual. This is not an exhaustive list. Report unusual symptoms to a healthcare professional.

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Common stacks

Peptides commonly paired with Motilin for synergistic effects.

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Sourcing & access

Research compound

Motilin is classified as a research compound. Regulatory status varies by jurisdiction. Always verify current legal status and source from vendors providing third-party certificates of analysis (COA).

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Frequently asked questions

Motilin is a 22-amino acid gastrointestinal hormone secreted by Mo/M cells of the upper small intestine during fasting. It is the primary regulator of the migrating motor complex, the coordinated GI contractions that clear the bowel between meals, released in approximately 100-minute cycles.

Motilin binds the motilin receptor (MLNR), a class A GPCR on smooth muscle cells and interstitial cells of Cajal. Activation via Gq/11 coupling increases intracellular calcium and triggers coordinated smooth muscle contraction, initiating phase III of the migrating motor complex.

Motilin peptide is a research compound with side effects including GI cramping at supraphysiologic doses, nausea, and abdominal discomfort. Erythromycin, a non-peptide motilin receptor agonist used off-label, has an established clinical safety profile but is limited by tachyphylaxis.

Motilin receptor agonists are being developed for diabetic gastroparesis and chronic intestinal dysmotility. Small-molecule agonists like mitemcinal and camicinal have advanced to Phase II trials with mixed results, while motilide compounds derived from erythromycin show clinical efficacy but are limited by tolerance development.

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Research references

  1. Motilin and its role in gastrointestinal motility: receptor pharmacologyPeeters TL, et al.Scand J Gastroenterol, 1999PubMed
  2. Motilin receptor agonists in gastrointestinal dysmotility and gastroparesisBroad J, Kung VW, et al.Gut, 2012PubMed
  3. Motilin signaling and the migrating motor complex: a physiological reviewDeloose E, Janssen P, et al.Neurogastroenterol Motil, 2012PubMed
  4. Motilin and erythromycin-like prokinetics: mechanism and clinical applicationTack J, et al.Curr Opin Pharmacol, 2022PubMed
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