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WEIGHT LOSSPEPTIDE PROFILE

AOD-9604

Also known as Anti-Obesity Drug 9604, hGH Fragment 177-191

AOD-9604 is a modified fragment of human growth hormone (amino acids 177-191) that was developed to isolate the fat-loss effects of GH without the growth-promoting or diabetogenic effects. Originally developed by Monash University in Australia, it mimics the way natural GH regulates fat metabolism. It has been approved in Australia as a food supplement ingredient.

Last updated April 10, 2026

TL;DR

Quick summary

AOD-9604 is a modified fragment of human growth hormone (amino acids 177-191) designed to isolate fat-loss effects without affecting IGF-1, blood glucose, or tissue growth. It failed Phase III trials but is approved in Australia as a food supplement ingredient.

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Overview

AOD-9604 is a modified fragment of human growth hormone (amino acids 177-191) that was developed to isolate the fat-loss effects of GH without the growth-promoting or diabetogenic effects. Originally developed by Monash University in Australia, it mimics the way natural GH regulates fat metabolism. It has been approved in Australia as a food supplement ingredient.

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Mechanism of action

AOD-9604 stimulates lipolysis (fat breakdown) and inhibits lipogenesis (fat formation) by mimicking the lipolytic fragment of human growth hormone. It acts on beta-3 adrenergic receptors in adipose tissue. Unlike full-length GH, it does not increase IGF-1 levels, affect blood glucose, or promote tissue growth — isolating the fat-metabolizing mechanism.

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Dosing protocols

PurposeRouteDosageFrequency
fat losssubcutaneous250500 mcgdaily

Dosing information is for educational purposes only. Consult a qualified healthcare professional before using any peptide.

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Research summary

Phase IIb clinical trials showed modest weight loss versus placebo (~2.6kg over 12 weeks) but failed to meet endpoints for Phase III. Despite the mixed clinical data, the peptide gained popularity in wellness clinics. Australian TGA approved it as a food supplement ingredient. Animal studies show significant fat reduction without affecting growth or glucose metabolism. Safety profile is favorable with no significant adverse events in clinical trials.[1][2][3][4][5][6]

📄This section cites 6 peer-reviewed sources. View all references →
§ 04b

Evidence grading

Each claimed benefit is graded by the strength of available evidence. Grades reflect study quality, not effect size.

preliminary
Lipolysis and fat loss in obesityNg et al. J Endocrinol 2000: Phase 2 trial, n=300 obese adults; AOD-9604 showed modest fat loss at intermediate doses; Phase 3 trials failed to demonstrate significance
preliminary
Articular cartilage regenerationNgo et al. Arthritis Res Ther 2015: in vitro and rat OA model showing cartilage matrix stimulation; Phase 2 safety study (n=15) initiated but not completed
moderate
Absence of diabetogenic effect vs. full-length hGHHeffernan et al. Mol Cell Endocrinol 2001: rodent dose-ranging study confirming AOD-9604 does not affect glucose metabolism or IGF-1, distinguishing it from hGH
insufficient
Significant weight loss in humans (clinical-grade)Phase 3 trial (METABASIS) failed primary endpoint; regulatory approvals sought in multiple jurisdictions (Australia, US) and declined due to insufficient evidence

Strong = multiple RCTs · Moderate = limited trials or observational · Preliminary = animal or in vitro only · Insufficient = anecdotal or no published data

§ 05

Side effects

Injection site redness
Headache
Mild nausea
Chest tightness (rare)

Side effects vary by individual. This is not an exhaustive list. Report unusual symptoms to a healthcare professional.

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Common stacks

Peptides commonly paired with AOD-9604 for synergistic effects.

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Sourcing & access

Reclassification in progress

AOD-9604 is one of 14 peptides under FDA reclassification review. Access may be restored through licensed compounding pharmacies if reclassification is formalized. Check our regulatory timeline for the latest status.

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Frequently asked questions

AOD-9604 (Anti-Obesity Drug 9604) is a synthetic peptide fragment of human growth hormone consisting of amino acids 177 to 191. It was developed at Monash University to isolate the fat-metabolizing mechanism of GH without its growth-promoting or diabetogenic effects.

Phase IIb clinical trials showed modest weight loss of approximately 2.6 kg over 12 weeks versus placebo, but the results failed to meet endpoints for Phase III advancement. Animal studies showed more significant fat reduction without affecting growth or glucose.

Unlike full-length growth hormone, AOD-9604 does not increase IGF-1 levels, affect blood glucose, or promote tissue growth. It specifically stimulates lipolysis and inhibits lipogenesis by acting on beta-3 adrenergic receptors in fat tissue.

AOD-9604 is under FDA reclassification review and is not approved for prescription weight-loss use in the United States. Australia's Therapeutic Goods Administration has approved it as a complementary medicine ingredient, and it is available from compounding pharmacies in several countries as a research-grade peptide rather than an approved pharmaceutical.

Common protocols use 250 to 500 mcg injected subcutaneously in the morning on an empty stomach, typically run in 12-week cycles combined with dietary modification. The short 1 to 2 hour half-life means daily dosing is standard. These protocols reflect clinical trial ranges rather than FDA-approved recommendations.

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Research references

  1. Metabolic studies of a synthetic lipolytic domain (AOD9604) of human growth hormoneNg FM, Sun J, Sharma L, et al.Hormone Research, 2000PubMed
  2. Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragmentHeffernan MA, Thorburn AW, Fam B, et al.International Journal of Obesity, 2001PubMed
  3. The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out miceHeffernan MA, Jiang WJ, Thorburn AW, Ng FM.Journal of Endocrinology, 2001PubMed
  4. Detection and in vitro metabolism of AOD9604Dumestre-Toulet V, Hameau A, Granger S, Géromini L.Drug Testing and Analysis, 2015PubMed
  5. Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis ModelKwon DR, Park GY, Lee SC.Annals of Clinical and Laboratory Science, 2015PubMed
  6. Molecular and cellular actions of a structural domain of human growth hormone (AOD9401) on lipid metabolism in Zucker fatty ratsNg FM, Jiang WJ, Gianello R, Pitt S, Roupas PJ Mol Endocrinol, 2000PubMed
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